“When scaling up a process based on adherent cells, a biopharmaceutical manufacturer has two options. Either stay in 2D, resulting in the use of heavy and difficult-to-handle multi-tray stacks with a ...

In this approach, researchers can simply transfect their preferred host cell—in most cases, human embryonic kidney (HEK) 293 cells—with all three plasmids in a stepwise procedure (see Figure 1).

3d). We also evaluated expression of EGFR in human embryonic kidney (HEK-293) cells. In this assay, we washed the cells with PBS to remove secreted EGFR extracellular domain in the culture medium ...