Antithrombin ligands and candidate receptor genes were co-expressed in HEK293T cells, and the candidate receptors bound to the ligand were identified by immunoprecipitation. The research is ...

We transiently transfected 293T cells in culture dishes (15-cm diameter) with pCMV-Myc-Dnmt1 (20 μg) or pcDNA3-HDAC1-F (10 μg) and collected cells 36 h post-transfection. We lysed cells in IPH ...

We infected human cells (293T or SupT1) with high-titer stocks of HIV-based vectors, then grew them for 30 days to insure that all the extrachromsomal forms of viral DNA were lost. We then ...