Over a century ago, a scientist in a New York laboratory peered at the cells he had cultivated in flasks and wondered ... or yeast can age and die, the culture continues to grow and is, in a ...

Department of Gastrointestinal Surgery, The Second Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, China Background: The high morbidity and mortality rates of colorectal ...

A total of 12 L culture was grown in batches of 2 L in 5 L flasks. After 4–5 h or an OD600 of >0.8, the incubator temperature was changed to 18 °C. The cultures were incubated overnight for at least ...

When the cells reached 80% confluence in T75 flasks, they were co-transfected with the lentiviral ... 200 μL of medium containing an anti-CD107a antibody. After 1 hour of co-culture, monensin was ...

“If you use a Google maps analogy,” explains Alex Ward, PhD, co-founder of Tolemy Bio. “Then we’re bypassing bottlenecks in ...

Researchers from the University of Pittsburgh have developed a new way to grow T cells in the lab that enables them to live ...

The most complex engineering of human cell lines ever has been achieved by scientists, revealing that our genomes are more resilient to significant structural changes than was previously thought.

Jan. 31, 2025 — Prolonged illnesses like cancer and chronic infections often leave the immune system in a state of exhaustion, where its frontline defenders -- T cells -- lose their ability to ...