We identified known factors, such as uracil DNA N-glycosylase (UNG), and unknown factors, such as the N6-adenosine methyltransferase, METTL3, as required to overcome floxuridine-driven cytotoxicity.

The RT-qPCR cycling conditions encompassed initial uracil-DNA glycosylase incubation for 2 min at 25°C, reverse transcription for 15 min at 50°C, activation of the Taq enzyme for 2 min at 95°C, and a ...

This involved the following steps: (1) Activation of Uracil-DNA glycosylase at 50°C for 2 min (performed once). (2) Activation of Dual-Lock™ DNA polymerase at 95°C for 2 min (performed once). (3) A ...

NASA iCOOL (Center for the Integration of the Origins of Life) and School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia 30332-0400, United States Institute of ...