and a uracil DNA glycosylase inhibitor. Base editing targeting Arg37 (R37X) exhibited one of the highest editing efficiencies (54%), accompanied only by silent bystander edits and minimal indel ...

We identified known factors, such as uracil DNA N-glycosylase (UNG), and unknown factors, such as the N6-adenosine methyltransferase, METTL3, as required to overcome floxuridine-driven cytotoxicity.

Libraries were partially treated with uracil-DNA glycosylase (UDG) and screened for ancient DNA damage patterns, high endogenous DNA content, and low contamination. We performed whole genome ...

The RT-qPCR cycling conditions encompassed initial uracil-DNA glycosylase incubation for 2 min at 25°C, reverse transcription for 15 min at 50°C, activation of the Taq enzyme for 2 min at 95°C, and a ...

NASA iCOOL (Center for the Integration of the Origins of Life) and School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia 30332-0400, United States Institute of ...

Figure 2. HBV replication and life-cycle in hepatocytes. Upon infection of hepatocytes, the HBV rcDNA is released into the nucleus and converted into cccDNA, which serves as the template for the ...