3T3 J-2 cell line derived from mouse embryonic fibroblast cells and can be used as feeder cells for growing keratinocytes. Buy Here!

3T3 cells are several cell lines of mouse embryonic fibroblasts. The original 3T3 cell line (3T3-Swiss albino) was established in 1962 by two scientists then at the Department of Pathology in the New York University School of Medicine, George Todaro and Howard Green.

3T3-J2 Irradiated Feeder Cells are optimized to support epithelial cell expansion in Conditional Reprogramming (CR) Medium.

This new protocol enables more rapid expansion of human epidermal keratinocytes in co-culture with not only 3T3-J2 cells but also other feeder cells including human dermal fibroblasts and human preadipocytes, two major alternatives to 3T3-J2 cells, with a long-term goal of developing customized skin autografts.

3T3-J2 are immortalized mouse fibroblasts used as feeders for human and mouse primary epithelial cells. In routine culture, they should be passaged every 3d day at 80-90 % confluency and split 1:6-1:10. Thawing and plating: Pre-warm 10 ml of medium, quickly thaw frozen cells and transfer into medium. Mix gently and spin 5 min at 1.000 rpm, RT.

In this Review, we focus on the application of cultured epithelial cells in regenerative medicine. In particular, we discuss the 3T3 mouse embryonic fibroblast (MEF) co‐culture system that has enabled stratified epithelial stem cell therapy for clinical use.

3T3-J2 Irradiated Feeder Cells are optimized to support epithelial cell expansion in Conditional Reprogramming (CR) Medium (Catalog #100-0352; Liu X et al. 2012; Liu X et al. 2017; Suprynowicz FA et al.).

Learn how to generate conditioned medium for epithelial cell expansion from 3T3-J2 Irradiated Feeder Cells and Conditional Reprogramming Medium.

Doubling time: ~18 hours (Kerafast=EF3003). Derived from site: In situ; Whole embryo; UBERON= UBERON_0000922. Cell type: Fibroblast; CL= CL_0000057. Breed/subspecies: Swiss albino. Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells.

3T3-J2 is a subclone of the original mouse embryonic fibroblast line, 3T3-Swiss Albino. After irradiation, these cells are commonly used as a feeder layer for growth of human keratinocytes. Species: Mus musculus, mouse Tissue: 12-13 day mouse embryos Morphology: Fibroblast Properties: Adherent Cytogenic data: