Dec 8, 2013 · By generating different MLKL mutants, we demonstrated that the plasma membrane localization of trimerized MLKL is critical for mediating necroptosis. Importantly, we found that the membrane...

Aug 24, 2018 · We demonstrate that intratumor administration of mRNA encoding MLKL elicits a potent antitumor T cell response—involving T cells directed against tumor neo-antigens—even in tumors that are...

Proliferating human keratinocytes and the SCC cell line A431 express GSDMA protein (80, 81), but its mRNA expression is strongly induced upon keratinocyte differentiation, suggesting a particularly important role in the outer epidermal layers .

Jan 1, 2025 · Recent studies have highlighted the significant role of necroptosis in tumorigenesis and tumor progression. Necroptosis is a controlled type of necrosis that relies on the phosphorylation of Mixed-lineage kinase-like protein (MLKL) by Receptor-interacting serine/threonine kinase 1 (RIPK1) and RIPK3 [187].

A unique neurodegenerative disease has been reported in association with a homozygous frameshift mutation, rs561839347, in MLKL that causes replacement of part of the C-terminal pseudokinase domain with a 21-residue sequence of random amino acids.

Jan 11, 2025 · In this study, we develop an azocalix [4]arene-modified supramolecular albumin nanoparticle, that co-delivers hydroxychloroquine and a mitochondria-targeting photosensitizer, designed to induce...

A-431 is a cell line exhibiting epithelial morphology that was isolated from the epidermis of an 85-year-old female patient with epidermoid carcinoma. This cell line is one of a series of cell lines established from solid tumors by D.J. Giard, et al. and can be used in cancer, toxicology, and immuno-oncology research.

Apr 20, 2021 · In this study, we aim to elucidate the molecular mechanism underlying hypoxic preconditioning that inactivates MLKL-dependent neuronal necroptosis after tGCI. Transient global cerebral ischemia was induced by the four-vessel occlusion method.

30161-1-AP targets IPMK in WB, IF/ICC, ELISA applications and shows reactivity with Human samples. PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Jan 22, 2016 · We show that mixed lineage kinase domain-like (MLKL) isoform 2, which lacks the pseudokinase domain and activation loop phosphorylation sites, is a more potent activator of cell death compared with MLKL isoform 1. Both MLKL isoforms are expressed in human monocyte-derived macrophages.