Amplified fragment length polymorphism (AFLP-PCR or AFLP) is a PCR-based tool used in genetics research, DNA fingerprinting, and in the practice of genetic engineering.

Amplified fragment length polymorphism (AFLP) is a PCR-based technique that uses selective amplification of a subset of digested DNA fragments to generate and compare unique fingerprints for genomes of interest.

Nov 8, 2017 · The AFLP technique is based on the selective PCR amplification of restriction fragments from a total digest of genomic DNA. How It Works. After final amplification, selectively amplified fragments are separated by gel electrophoresis and visualized autoradiographically.

AFLP is a highly sensitive method for detecting polymorphism in DNA. AFLP is a dominant type of molecular marker, it involves restriction followed by PCR amplification of genomic DNA. Primers and adapters are used for pre-selective and selective PCR amplifications.

A novel DNA fingerprinting technique called AFLP is described. The AFLP technique is based on the selective PCR amplification of restriction fragments from a total digest of genomic DNA. The technique involves three steps: (i) restriction of the DNA and ligation of oligonucleotide adapters, (ii) selective amplification of sets of restriction ...

Amplified Fragment Length Polymorphism is a nucleic acid finger printing method to find out genetic variation that exists between closely related genes. This method was de­signed by Zabeau and Vos (1993) and Vos et al. (1995).

How would you distinguish organisms based on their genomes? One of the tools scientists use is amplified fragment length polymorphism polymerase chain reaction (AFLP-PCR).

AFLP or amplified fragment length polymorphism is a PCR-based molecular technique that uses selective amplification of a subset of digested DNA fragments from any source to generate and compare unique fingerprints of genomes.

AFLP is a versatile technique for genome-wide screening of genetic diversity and can be applied to almost any organism. The technique relies on detecting genetic polymorphisms through differential endonuclease restriction digestion of genomic DNA.

May 31, 2007 · The AFLP technique is a powerful DNA fingerprinting technology applicable to any organism without the need for prior sequence knowledge. The protocol involves the selective PCR amplification of...