Mix-and-read biochemical HTS AMPK Assay. Measure AMPK Enzyme Activity with an FP, FI, or TR-FRET Readout. Services also available.

AMP‐activated protein kinase (AMPK) exhibits a key role as a master regulator of cellular energy homeostasis (1). AMPK exists as a heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits. Binding of AMP to the γ subunit allosterically activates the complex.

Oct 31, 2022 · Recent advances revealed several non-canonical pathways for AMPK activation, including roles of calcium, nutrients and metabolites, as well as DNA and lysosome damage, providing novel insights ...

4 days ago · Validation of the proposed AMPK activity assay. (A) OD values from the ELISA following the kinase reactions (40-min reaction time) with different amounts of AMPK α1β1γ1 (0, 5, 10, 20, 40, 60, 80 or 100 ng per reaction), n = 4. OD values from the condition of 0 ng AMPK were considered as blank values and subtracted from all the OD values in ...

In this review, we provide a comprehensive summary of both indirect and direct AMPK activators and their modes of action in relation to the structure of AMPK.

Nov 4, 2016 · We summarize three different expression systems for AMPK production: bacteria, insect cells and mammalian cells. In addition, kinase activity measurement, kinase substrate identification and determination of physical interaction with AMPK are discussed.

Together, these three mechanisms of activation can cause up to a 1000-fold increase in AMPK activity, and this chapter will describe assays to monitor these different activation mechanisms in cell-free assays. Adenine nucleotides (AMP/ADP/ATP) bind to sites formed by sequence repeats termed CBS motifs in the AMPK-γ subunits.

Feb 25, 2025 · In Subheading 3.1 of this chapter, we describe assays to assess AMPK activity in cell lysates, and the use of cells expressing mutant AMPK complexes that are insensitive to AMP or to ADaM site activators, in order to test the mechanism of action on any novel ligand that activates AMPK in intact cells.

AMP-activated protein kinase (AMPK) is the master regulator of cellular and organismal energy homeostasis, playing an essential role in modulating metabolism and other cellular processes. ... (ELISA)-based AMPK activity assay with simple steps and high sensitivity. This assay offers a robust, in-house alternative to the traditional radioactive ...

Aug 21, 2024 · Here we report an enzyme-linked immunosorbent assay (ELISA)-based AMPK activity assay with simple steps and high sensitivity, which represents an alternative in-house method to the traditional radioactive method as well as other techniques that require special reagents and commercial reagent kits.