Apr 27, 2012 · Using an approach combining biochemistry, single-cell imaging and computational modeling, we here demonstrate that excitotoxic injury activated the bim promoter in a FOXO3-dependent manner. The...

Jun 7, 2020 · Our findings suggest that AMPK participates in the regulation of FOXO3a-mediated neuronal apoptosis in the developing rat brain after HI. Agents targeting AMPK may offer promise for rescuing neurons from HI-induced damage.

Jul 10, 2009 · Our study shows that FOXO3 reduces intracellular ROS levels by directly inducing the antioxidant Trx. When activated by AMPK, FOXO3 directly binds to the Trx promoter and forms a transcriptional complex on the Trx promoter, which may …

AMPK is a heterotrimeric complex composed of three subunits: A catalytic subunit (α) and two regulatory subunits (β and γ). Activation of AMPK occurs in response to an increased cellular AMP/ATP ratio, which arises during cellular metabolic stress [38,39,40].

Jul 1, 2023 · The regulation of FoxO3 by AMPK may play a crucial role in mitochondrial gene expression that controls energy balance and confers resistance to mitochondria-associated ferroptosis in vitro and in vivo.

The activation of AMPK reduced AKT activation, and led to dephosphorylation and nuclear translocation of FOXO3. Subsequent mutation studies indicated that bim gene activation during excitotoxic injury required direct FOXO3 phosphorylation by AMPK …

Multiple signalling pathways, including AMP-activated protein kinase (AMPK), are indicated to promote autophagy in ischaemic/hypoxic (I/R) heart. However, it's far more to clarify the orchestrated cross-talk between AMPK and other signalling pathways in the autophagy.

Oct 12, 2007 · Here, we find that AMPK directly regulates mammalian FOXO3, a member of the FOXO family of Forkhead transcription factors known to promote resistance to oxidative stress, tumor suppression, and longevity. We show that AMPK phosphorylates human FOXO3 at six previously unidentified regulatory sites.

The regulation of FoxO3a by AMPK may play a crucial role in mitochondrial gene expression that controls energy balance and confers resistance to mitochondria-associated ferroptosis and CIR injuries.

May 15, 2018 · In the present work, we provided new insights concerning the implication of the energy sensor AMPK in the regulation of FOXO3 pathway. In addition to an increase in messenger levels, the present study reveals that AMPK stabilizes FOXO3 protein and has a potent role in Mul1 mitophagic axis.