Apr 10, 2020 · Here, we describe acyl resin-assisted capture (Acyl-RAC), a recently developed method based on selective capture of endogenously S-acylated proteins by thiol-reactive Sepharose beads.

Here, we describe acyl resin-assisted capture (Acyl-RAC), a recently developed method based on selective capture of endogenously S-acylated proteins by thiol-reactive Sepharose beads.

Acyl-RAC方法能够鉴定出任何蛋白的硫醇上的修饰情况，并且经常被误用为验证S-脂酰化蛋白的方法，如果要鉴定新的S-脂酰化蛋白，建议使用Acyl-RAC和alk-16（+/-NH2OH）结合的方法。 详细可联系我们所要原文，参考详细的实验protocol。

We recently described a simple and robust methodology to rapidly identify S-nitrosylation sites in proteins via resin-assisted capture (RAC) and provided an initial description of the applicability of the technique to S-acylated proteins (acyl-RAC).

Feb 14, 2024 · Acyl-biotin exchange (ABE), acyl resin-assisted capture (acyl-RAC) and metabolic labelling reveal whether a protein can be acylated. Use of hydroxylamine controls ensures that the detected...

We recently described a simple and robust methodology to rapidly identify S-nitrosylation sites in proteins via resin-assisted capture (RAC) and provided an initial description of the applicability of the technique to S-acylated proteins (acyl-RAC).

Jun 12, 2017 · Acyl-resin-assisted capture (acyl-RAC) is an alternative technique which has been described recently 21 and was derived from a modification of the biotin switch assay used...

May 12, 2021 · The S-acylation levels were detected by an acyl-resin capture (acyl-RAC) assay. Palm- palmitoylated protein, Depalm- depalmitoylated protein.

We recently described a simple and robust methodology to rapidly identify S-nitrosylation sites in proteins via resin-assisted capture (RAC) and provided an initial description of the applica- bility of the technique to S-acylated proteins (acyl-RAC).

Apr 10, 2020 · Acyl-RAC (Acyl-Resin Assisted Capture) is a highly sensitive, reliable and easy to perform method to detect reversible lipid modification of cysteine residues (S-acylation) in a variety of biological samples.