One widely accepted method for identifying cancer stem cells is based on the enzymatic activity of aldehyde dehydrogenase 1 (ALDH1), 2-7 a detoxifying enzyme responsible for the oxidation of intracellular aldehydes. 1 There are different isoforms of ALDH.

Nov 25, 2016 · We found that non-small-cell lung cancer (NSCLC) cells express high levels of multiple aldehyde dehydrogenase (ALDH) isoforms via an informatics analysis of metabolic enzymes in NSCLC and...

This novel finding suggests that multiple ALDH isoforms contribute to ALDEFLUOR activity in colon cancer stem cells and decreases in ALDEFLUOR-positive stem cells at high cell density are due to decreased expression of multiple ALDH isoforms.

Trypsin resistance of ALDHs synthesized in the TNT coupled reticulocyte lysate. Each precursor and mature protein was translated for 60 min at 30°C. An aliquot was then removed and incubated at 15°C for the indicated times.

Sep 25, 2017 · Adherent A549 cells were then harvested with trypsin containing 0.25% EDTA (Gibco; Thermo Fisher Scientific, Inc.) at a concentration of 2×10 5 viable cells for each sample, and were applied in the detection of the enzymic activity of ALDH.

Mar 14, 2024 · In this chapter, it is described how CSCs are detected and isolated by using ALDH activity assay. Aldehyde dehydrogenases (ALDHs) are a family of enzymes that catalyze the oxidation of endogenous aldehyde substrates to their corresponding carboxylic acids and conversion of retinol to retinoic acid.

Jan 11, 2016 · Our results demonstrate that trypsin-sensitive (TS) breast and colon cancer cells subpopulations show increased ALDH activity, higher ability to exclude Hoechst 33342, enlarged proportion of...

One widely accepted method for identifying cancer stem cells is based on the enzymatic activity of aldehyde dehydrogenase 1 (ALDH1),2-7 a detoxifying enzyme responsible for the oxidation of intracellular aldehydes.1 There are different isoforms of ALDH. The ALDEFLUOR® assay system has been developed to detect the activity of the ALDH1 isoform.

Apr 1, 2024 · In this study, we performed three rounds of rational design to address these issues. Specifically, the surface digestive sites of pepsin and trypsin were replaced with other polar amino acids, while hydrophobic amino acids were incorporated to reshape the catalytic cavity of ALDH2.

Jul 3, 2024 · Our work reveals that the ALDH1A3-ACSS2 couple directly coordinates nuclear acetaldehyde-acetyl-CoA metabolism with specific chromatin-based gene regulation and represents a potential therapeutic vulnerability in melanoma.