Jun 16, 2023 · Here, we present an optimized ATAC-seq protocol to elucidate chromatin accessibility changes at the epigenetic level in head and neck squamous cell carcinoma cells. We describe steps for cell lysate preparation, transposition, and tagmentation, followed by library amplification and purification.

Jan 1, 2025 · ATAC-seq (Assay for Transposase-Accessible Chromatin using sequencing) is a method to study genome accessibility. It enables researchers to identify regions of the genome accessible to transcription machinery—indicating potential transcriptional activity—and regions that are condensed and transcriptionally inactive.

Oct 26, 2020 · Here we describe an updated and optimized protocol for ATAC-seq, called Omni-ATAC, that is applicable across a broad range of cell and tissue types. The ATAC-seq workflow has five main steps:...

The method is a fast and sensitive alternative to DNase-seq for assaying chromatin accessibility genome-wide, or to MNase-seq for assay- ingnucleosome positions inaccessible regions of thegenome.

Jun 9, 2020 · We describe here a protocol for the generation of sequence-ready libraries for population epigenomics studies, and the analysis of alignment results. We show that the protocol can be used to monitor chromatin structure changes in populations when exposed to environmental cues.

ATAC-seq (Assay for Transposase-Accessible Chromatin using sequencing) is a technique used in molecular biology to assess genome-wide chromatin accessibility. [1] In 2013, the technique was first described as an alternative advanced method …

ATAC-seq (Assay for Transposase-Accessible Chromatin with high-throughput sequencing) is a method for determining chromatin accessibility across the genome. It utilizes a hyperactive Tn5 transposase to insert sequencing adapters into open chromatin regions.

ATAC-Seq detects open-chromatin regions and maps transcription factor binding events genome-wide by means of direct in vitro transposition of native chromatin.

This unit describes Assay for Transposase Accessible Chromatin with high-throughput sequencing (ATAC-seq), a method for mapping chromatin accessibility genome-wide. This method probes DNA accessibility with hyperactive Tn5 transposase, which inserts ...

The transposase-accessible chromatin using sequencing (ATAC-seq) offers a simplified approach to detect chromatin changes in cancer cells after genetic intervention and drug treatment.