ATAC-seq (Assay for Transposase-Accessible Chromatin using sequencing) is a technique used in molecular biology to assess genome-wide chromatin accessibility. [1] In 2013, the technique was first described as an alternative advanced method …

Jan 5, 2015 · This unit describes Assay for Transposase-Accessible Chromatin with high-throughput sequencing (ATAC-seq), a method for mapping chromatin accessibility genome-wide. This method probes DNA accessibility with hyperactive Tn5 transposase, which inserts sequencing adapters into accessible regions of chr …

Oct 26, 2020 · Here we describe an updated and optimized protocol for ATAC-seq, called Omni-ATAC, that is applicable across a broad range of cell and tissue types. The ATAC-seq workflow has five main steps:...

Feb 9, 2021 · The ATAC-Seq method was just published in 2013 and it has already become one of the most common and powerful ways to study chromatin states on a genome-wide level. This article covers what ATAC-Seq is and how to use it in your research.

The assay for transposase-accessible chromatin with sequencing (ATAC-Seq) is a popular method for determining chromatin accessibility across the genome. By sequencing regions of open chromatin, ATAC-Seq can help you uncover how chromatin packaging and other factors affect gene expression.

Jan 1, 2025 · ATAC-seq (Assay for Transposase-Accessible Chromatin using sequencing) is a method to study genome accessibility. It enables researchers to identify regions of the genome accessible to transcription machinery—indicating potential transcriptional activity—and regions that are condensed and transcriptionally inactive.

Feb 3, 2020 · Here, we discuss the major steps in ATAC-seq data analysis, including pre-analysis (quality check and alignment), core analysis (peak calling), and advanced analysis (peak differential analysis and annotation, motif enrichment, …

ATAC-seq identifies open chromatin regions, ChIP-seq verifies and accurately locates transcription factor binding sites, and RNA-seq evaluates changes in gene expression. Such detailed analyses can illuminate the connections between chromatin accessibility, transcription factor interaction, and downstream target gene regulation, providing ...

A recommended practical technique for studying the openness of chromatin is the assay for transposase-accessible chromatin with high throughput sequencing (ATAC-Seq). The idea is to employ the transposase Tn5 to quickly connect to open chromatin, but Tn5 lacks the capability to …

Here, we discuss the major steps in ATAC-seq data analysis, including pre-analysis (quality check and alignment), core analysis (peak calling), and advanced analysis (peak differential analysis and annotation, motif enrichment, footprinting, and nucleosome position analysis).