To generate large numbers of activated human B cells in vitro, we developed a B-cell culture system in which B-cell populations are expanded on feeder cells that express low levels of CD154 in medium containing the recombinant human cytokines IL-2, IL-4, IL-21, and BAFF .

Get tips and tricks on how to isolate and expand human B cells immediately ready for downstream applications, including studies on B cell development, signaling, proliferation and differentiation as well as vaccine development and B cell engineering.

Nov 15, 2016 · We developed a culture method to support the efficient activation and proliferation of naive and memory human B cells. This culture supports extensive B cell proliferation, with ∼10 3-fold increases following 8 d in culture and 10 6-fold increases when cultures are split and cultured for 8 more days. In culture, a significant fraction of ...

Memory and plasma B cells produce antibodies including immunoglobulin (Ig) IgM, IgG, and IgE. This page describes the development of mature B cell types and tools to study B cells including cell culture, immunoassays, and cell markers for immunophenotyping.

We used the B Cell Culture and Expansion Kit from Humimmu. It works well for two weeks culture. I would suggest that you use negative selection for the purification of your cells. There are...

Here, we describe a minimalistic in vitro culture system that supports efficient differentiation of human naive B-cells into antibody-secreting cells.

Alternatively, B cell culture conditions have been refined to: (i) either maintain antibody-secreting B cells long enough in culture to screen their secreted antibodies for specificity or function; or (ii) culture times have been minimized by enrichment of antigen-specific B cells.

In this review, we summarize our studies to the regulation of the replicative life span, differentiation, and maturation of B cells that led to the development of a platform that uses immortalization of human B cells by in vitro genetic modification for antibody development.

Apr 9, 2021 · Here we describe a detailed protocol for the purification and ex vivo expansion of primary, nonmalignant human GC B cells. We present methodology for the high-efficiency transduction of these...

Sep 1, 2021 · Stable, long-term culture of primary B lymphocytes has many potential scientific and medical applications, but remains an elusive feat. A major obstacle to long-term culture is that in vitro mitogens quickly drive B cells to differentiate into short-lived plasma cells (PCs).