Baby Hamster Kidney fibroblasts (BHK cells) are an adherent cell line used in molecular biology. The cells were derived in 1961 by I. A. Macpherson and M. G. P. Stoker. Nowadays, subclone 13 is occasionally used, which was originally derived by single-cell isolation from the kidneys of five unsexed, 1-day-old hamsters .

BHK-21 [13] fibroblasts were isolated from the kidney of an uninfected golden hamster. The cells can be used for the routine diagnosis of rabies.

Nov 13, 2015 · Baby Hamster Kidney Fibroblast Cells (BHK-21 Line) The BHK-21 fibroblast cell line was established in March of 1961 by I. A. Macpherson and M. G. P. Stoker. The widely used line is a subclone (clone 13) of a parental line derived from …

Use the procedures below to initiate and maintain a culture of BHK cells. • All solutions and equipment that come in contact with the cells must be sterile. • Always use proper sterile technique and work in a laminar flow hood.

Baby hamster kidney cells (bhk -21) are the most commonly used continuous cell lines for the production of vaccines for animals.

The BHK21 cell line was established in 1961 from the kidneys of 5 Syrian hamsters from litter number 21. Since this time, this cell line has been a laboratory standard for the growth of countless viruses and the study of many biological processes.

Baby Hamster Kidney Fibroblast Cells (BHK-21) The BHK-21 fibroblast cell line was established in March of 1961 by I. A. Macpherson and M. G. P. Stoker. The widely used line is a subclone (clone 13) of a parental line derived from the kidneys of five unsexed, 1-day-old hamsters.

Baby hamster kidney (BHK) cells are most commonly used in the production of animal products, the most important of which is the production of a vaccine against Foot-and-Mouth Disease and rabies [8].

BHK-21 cells are the most commonly used cells for vaccine production. Not all cell lines can be adapted to suspension growth. In general, anchorage-dependent cells (must be attached to a substrate to grow) will grow in suspension only with the use of microcarrier beads.

In the present study, the BHK-21 (C5) was characterized, and it was tried to determine the identity of BHK-21 (C5) as a continuous cell line by Polymerase chain reaction (PCR) molecular procedure in Iran.