Mar 21, 2017 · This single chain camel antibody specifically recognizes F-actin and has been successfully employed to study actin dynamics in diverse eukaryotes. We generated two expression vectors for Cb, where Cb is fused to either Emerald GFP (Cb-EmGFP; [Shaner et al., 2007]) or Halo (Cb-Halo; [Los et al., 2008]).

Sep 13, 2019 · We expressed F-actin binding nanobodies (Cb) fused to a variety of tags including GFP-emerald (Cb-Emerald FP), photoconvertible mEos3.2 28 (Cb-mEos3.2) and SNAP-tag 29 to visualise MIC2 and F ...

Mar 21, 2017 · Cb labels filamentous actin structures within the parasite cytosol and labels an extensive F-actin network that connects parasites within the parasitophorous vacuole and allows vesicles to be exchanged between parasites.

Rab11A-positive vesicles (red) in close contact with dynamic cytosolic actin filaments in intracellular T. gondii parasites expressing Cb-Emerald GFP (green). Imaging speed: 2 fps.

T. gondii is a protozoan parasite that infects humans and food animals and causes worldwide zoonosis. The only definitive hosts are members of the family Felidae, and humans are …

Approximately 85 percent of women of childbearing age in the United States are susceptible to acute infection with the protozoan parasite Toxoplasma gondii. Transmission of T. gondii to the...

Oct 4, 2019 · To analyse the role of F‐actin during merozoite invasion, we expressed CB‐Halo and Cb‐EmeraldFP in P. falciparum and validated their efficiency to analyse F‐actin localisation and dynamics in this parasite (see also 25).

Aug 1, 2017 · The Toxoplasma Cb-actin line also revealed a second pool of F-actin inside intracellular and extracellular tachyzoites. It was detected as discrete entities that nevertheless remained discernible from the diffuse cytoplasmic signal possibly caused by the weak binding of Cb-actin to G-actin.

Congenital toxoplasmosis occurs when maternal infection is acquired for the first time in pregnancy.

Unlike skeletal muscle actin, Toxoplasma filaments intrinsically undergo rapid treadmilling due to a high critical concentration, fast monomer dissociation, and rapid nucleotide exchange.