
Engineering Artificial Antigen-presenting Cells to Express a …
To facilitate the therapeutic application of antigen-presenting cells (APCs), we have developed a cell-based artificial APC (aAPC) system by engineering K562 cells with lentiviruses to direct the stable expression and secretion of a variety of co-stimulatory molecules and cytokines.
Expression of Functional CD32 Molecules on Human NK Cells Is …
Apr 1, 1998 · K562 cell line or Jurkat cells transfected with full-length cDNAs encoding FcγRIIb1, FcγRIIc1, and FcγRIIc3 were analyzed by indirect immunofluorescence staining with four anti-CD32 MoAbs (filled histograms) or with a mouse IgG isotype control (dotted line), followed by staining with FITC-GAMIgG.
Human cell-based artificial antigen-presenting cells for cancer ...
Jan 1, 2014 · Although strongly positive for CD32 expression, K562’s CD32-mediated endocytotic activity is relatively weak. When CD64 was introduced along with the FcR common γ subunit, however, K562 acquired efficient endocytotic activity mediated via CD64, enabling an uptake of a soluble antigen/antibody complex and processing and presentation of CD4 ...
Here CD32 that binds to a crystallizable fragment of IgG monoclonal antibody was genetically expressed on human K562 leukemia cells to provide a ligand for T-cell receptor. CD86 and 4-1BBL,...
Lentiviral-mediated expression of CD64 and CD32 in K562 …
The parental K562 cell line was transduced with a lentiviral vector expressing CD64 or CD32, and the cells were sorted and cultured as described in Materials and Methods.
Flow cytometry of cell surface markers of K562 and K562-IR …
Tyrosine kinase inhibitor (TKI) resistance is a major problem in chronic myeloid leukemia (CML). We generated a TKI-resistant K562 sub-population, K562-IR, under selective imatinib-mesylate...
A panel of human cell-based artificial APC enables the expansion …
In this article, we shifted focus to CD4 + T cells and developed a panel of novel K562-derived aAPC, where each expresses a different single HLA-DR allele, invariant chain, HLA-DM, CD80, CD83 and CD64; takes up soluble protein by endocytosis and processes and presents CD4 + …
To facilitate the therapeutic application of antigen- presenting cells (APCs), we have developed a cell-based artificial APC (aAPC) system by engineering K562 cells with lentiviruses to direct the stable expression and secretion of a variety of co-stimulatory molecules and cytokines.
A cell-based artificial antigen-presenting cell coated with anti-CD3 ...
We compared the ability of two genetically modified myeloid cells, K562 and U937, to serve as artificial antigen-presenting cells (aAPC). Both aAPC were stably transfected with the low-affinity Fcgamma receptor CD32 (K32/U32 cells).
Engineering Artificial Antigen-presenting Cells to Express a …
May 1, 2007 · K562 cells (American Type Culture Collection, Manassas, VA) were transduced with the lentiviral vector CD32-pCLPS or CD64-pCLPS, and cells with high expression were cloned using high-speed MOFLO sorting (Cytomation, Fort Collins, CO) to generate CD32-transduced or CD64-transduced aAPCs.