
CFU-GEMM - Wikipedia
In current terminology, CFU-S refers to the pluripotent stem cells that can differentiate into all types of blood cells. CFU-S divides into two lineages: the lymphoid precursor (CFU-LSC) and the myeloid precursor (CFU-GEMM).
Culture and Analysis in Hematopoietic CFU Assays - STEMCELL
The colony-forming unit (CFU) assay is one of the most widely used assays for hematopoietic stem and progenitor cells (HSPCs). CFU assays allow measurement of the proliferation and differentiation ability of individual cells within a sample.
Current practices and Prospects for Standardization of the ...
The CFU assay takes advantage of a hematopoietic progenitor’s ability to proliferate and differentiate to form a colony of cells committed to specific blood cell lineages.
CFU-E - Wikipedia
The term CFU-E (Colony Forming Unit-Erythroid [1]) denotes a hematopoietic stem cell in the bone marrow which eventually matures into a red blood cell (also called an erythroid cell). It arises from CFU-GEMM (via BFU-E, [2] which stands for "erythroid burst-forming units" [3]) and gives rise to proerythroblasts.
• Develops in medium containing EPO and other cytokines, particularly interleukin-3 (IL-3) and stem cell factor (SCF) Are generally larger and have more clusters in cord blood than in bone marrow or (mobilized) peripheral blood. CFU-E . (Colony-forming unit − erythroid) • A colony containing 8 – 200 erythroblasts in one to two small clusters.
Isolation and transcriptome analyses of human erythroid progenitors ...
Dec 4, 2014 · We developed a flow cytometry-based strategy for isolating human BFU-E and CFU-E cells based on the changes in expression of cell surface markers during in vitro erythroid cell culture. BFU-E and CFU-E are characterized by CD45 + GPA − IL-3R − CD34 + CD36 − CD71 low and CD45 + GPA − IL-3R − CD34 − CD36 + CD71 high phenotypes, respectively.
CFU-Meg - Wikipedia
In order to eventually produce a megakaryocyte, the haematopoietic stem cell must generate myeloid cells, so it becomes a common myeloid progenitor, CFU-GEMM. This in turn develops into CFU-Meg, which is the colony forming unit that leads to the production of megakaryocytes.
CFU-S assay: a historical single-cell assay that offers modern …
The CFU-S assay indicated the short-term kinetics of erythropoietic output potential and revealed that erythroid cell generation is a clonal feature of all these populations (but not of GMPs), whereas LTRA allowed investigation of their long-term kinetics of peripheral blood reconstitution.
Evaluation of post-thaw CFU-GM: clinical utility and role in quality ...
To investigate the impact of post-thaw CFU-GM counts on the quality of umbilical cord blood (UCB), we studied transplant outcomes in 269 patients receiving single UCB transplant. We also correlated the post-thaw CFU-GM counts of 1912 units with the pre-freeze and post-thaw graft characteristics, hoping to optimize selection criteria of UCB.
Measuring Cord Blood: Learn What TNC, CD34, & Other Counts
Oct 2, 2023 · One study found that the number of colony-forming units (CFUs) was found to be a stronger independent predictor of engraftment and a potential better option in assessing the potency of a cord blood unit for transplantation. Measuring the number of colony-forming units is direct. A sample of the cells is placed on growth media and into an incubator.