We compared the concentrations of stromal clonogenic cells, colony forming units–fibroblast (CFU-Fs), in norm and pathology. Initially, culture conditions were optimized by demonstrating that fetal bovine serum heat inactivation could significantly repress colony formation.

The fibroblastic nature of CFU-F progeny is demonstrated by immunofluorescent staining with anti-type I and anti-type III collagen, which are components of the marrow intercellular space and known to be produced by fibroblasts.

Aug 1, 1980 · The present report describes a suspension culture technique for cloning human bone marrow fibroblasts, with which we were able to determine the physical characteristics and proliferative status of fibroblast colony-forming cells (CFU-F) and the antigenic characteristics of CFU-F and their fibroblast progeny.

H Castro-Malaspina, RE Gay, G Resnick, N Kapoor, P Meyers, D Chiarieri, S McKenzie, HE Broxmeyer, MA Moore; Characterization of human bone marrow fibroblast colony-forming cells (CFU-F) and their progeny.

A standard liquid culture system was developed to isolate MSC by their adherence to the plastic of tissue-culture plates, where clonal populations expand from single precursors—colony-forming-unit fibroblasts (CFU-F).

Fibroblastic colony forming units (CFU-F) were discovered as plastic-adherent clonogenic fibroblast-like cells in monolayer bone marrow cultures. It was hypothesized that CFU-F are formed by a small number of precursors evenly distributed among the initial cell population of bone marrow culture.

In patients with MF, marrow fibroblast colony (CFU-F) formation could not be studied; fibroblasts were grown from marrow explants. CFU-E from normals and MPD patients exhibited similar cell density distribution and similar cell sedimentation rates.

We compared the concentrations of stromal clonogenic cells, colony forming units-fibroblast (CFU-Fs), in norm and pathology. Initially, culture conditions were optimized by demonstrating that fetal bovine serum heat inactivation could significantly repress colony formation.

Characterization of human bone marrow fibroblast colony-forming cells (CFU-F) and their progeny Blood. 1980 Aug;56(2):289-301. Authors H Castro-Malaspina, R E Gay, G Resnick, N Kapoor, P Meyers, D Chiarieri, S McKenzie, H E Broxmeyer, M A …

The bone marrow population includes a cell type which is able to give rise in vitro to fibroblast colonies (CFU-F). CFU-F are adherent, nonphagocytic, nonproliferating, relatively radioresistant, Ia antigen-negative cells having a wide density and sedimentation rate distribution.