The regulatory function of a core fragment (cC) from human cytomegalovirus (CMV) promoter was investigated with cell-lineage specificity in NIH3T3 (fibroblast) and hematopoietic cell lines U937 (monocyte/macrophage), LCL (lymphoid), DAMI (megakaryocyte), and MEL (erythroid).

Sep 18, 2024 · The expression of GFP under the CMV promoter remained stable for up to 20 days. The induction of 3T3-L1 differentiation in three days after AAV transduction did not alter the GFP expression level, and AAV2/6 showed the best transduction efficiency.

Here we show that the mouse cytomegalovirus (MCMV) m166 protein restricts cell surface expression of the TRAIL death receptor in infected cells, thus protecting them from TRAIL mediated apoptosis.

Results: The cC element in reverse-orientation not only boosted upstream Es promoter to levels comparable to full-length EF in DAMI, U937 and 3T3 cells, but also blocked the suppression of downstream Sv promoter by Es in U937 and 3T3 cells with …

In this study, we used genetic constructs under control of the promoters—strong cytomegalovirus promoter (pCMV), constitutive cell promoter of the PCNA gene (pPCNA), and the potentially fibroblast-specific promoter of the IGFBP2 gene (pIGFBP2) for genetic modification of fibroblasts.

Dec 12, 1999 · We used recombinant murine cytomegalovirus (mCMV) expressing either green fluorescent protein (GFP) or β-galactosidase under control of human elongation factor 1 promoter or CMV immediate early-1 promoter as reporter genes for infected brain cells.

NIH3T3 cells have been widely used in biomedical research. NIH3T3/GFP (CMV) stable cells express GFP under the control of the CMV promoter. This stable cell line is suitable for in vitro and in vivo imaging.

Feb 1, 2004 · In this report, we evaluated the use of lentivirus-mediated gene transfer into 3T3-L1 mature adipocytes. We demonstrate that quiescent, fully differentiated 3T3-L1 adipocytes as well as 3T3-L1 preadipocytes can be efficiently transduced with HIV-1-derived lentiviral vectors.

Mar 10, 2011 · In somatic NIH 3T3 cells, the γ-retroviral vector LMP and bidirectional CMV construct PCG(Bi) produced the strongest (60%) reduction in firefly luciferase activity as compared with all other ...

Jan 25, 2021 · Our study provides evidences for the immediate and efficient knockdown of C/EBPβ protein to understand time-dependent preadipocytes differentiation. Keywords: C/EBPβ, protein transfection, adipogenesis, 3T3-L1, dedifferentiation, obesity.

On the basis of recent studies demonstrating the chromatin insulating properties of divergent promoters, we sought to develop a bidirectional lentiviral vector with which to conduct RNA...

Expression levels of CMV and GFAP promoters in non-glial and glioblastoma cell lines. (A) Three cell lines-NIH-3T3, N2A, and U251-were transiently transfected with pCMV-HSVtk-P2A-EGFP or...

BALB/c-3T3 cells express only the predominant immediate early (IE) nuclear phosphoproteins (IE68 or IE94) of human CMV (HCMV) or simian CMV (SCMV) and do not replicate the input viral genomes.

Jan 1, 1996 · The mCMV was the most active in dihydrofolate reductase-deficient Chinese hamster ovary (CHO dhfr−) cells and BALB/3T3 clone A31 mouse embryo cells. The h2CMV was more active than the other promoters in Bowes human melanoma cells and in Vero African green monkey kidney cells.

(A) 3T3-L1 preadipocytes were transduced by CMV-GFP lentivirus at 20, 40, 80, or 200 ng p24 per 10 5 cells or transfected with 0.1, 0.25, 0.5, or 1 A g of pLV-CMV-GFP plasmid using JetPEI...

Aug 2, 2019 · In this study, RNA sequencing and small RNA sequencing were used to explore alterations in mRNAs and miRNAs in E6 over-expressing NIH/3T3 cells (NH-E6) compared with control cells (NH-GFP).

In this report, we evaluated the use of lentivirus-mediated gene transfer into 3T3-L1 mature adipocytes. We demonstrate that quiescent, fully differentiated 3T3-L1 adipocytes as well as 3T3-L1 preadipocytes can be efficiently transduced with HIV-1-derived lentiviral vectors.

Dec 15, 1999 · We used recombinant murine cytomegalovirus (mCMV) expressing either green fluorescent protein (GFP) or β-galactosidase under control of human elongation factor 1 promoter or CMV immediate early-1 promoter as reporter genes for infected brain cells.

Apr 3, 2023 · Our results reveal that CMV infection prevents TNF-induced necroptosis in cardiomyocytes; however, the opposite phenotype is observed in cardiac fibroblasts. CMV infection also suppresses...