Dec 4, 2019 · CRISPR-Cas systems have well characterized, modular structures. Here the authors use that architecture to design a Cas12a library of 560 synthetic chimeras, with altered PAM preferences and...

Jan 11, 2025 · We designed multiplex sgRNA-A9 chimera targeting the ASCL1 gene for activation with QCP-p65-HSF1, while another sgRNA-MS2 chimera simultaneously targeting the XIST1 gene for repression with ...

Apr 10, 2019 · Here we describe CHIME: CHimeric IMmune Editing, a CRISPR-Cas9 bone marrow delivery system to rapidly evaluate gene function in innate and adaptive immune cells in vivo without ex vivo...

Mar 17, 2022 · A compact protein with a size of <1,000 amino acids, the CRISPR-associated protein CasX is a fundamentally distinct RNA-guided nuclease when compared to Cas9 and Cas12a. Although it can induce RNA-guided genome editing in mammalian cells, the activity of CasX is less robust than that of the widel …

Mar 17, 2022 · A compact protein with a size of <1,000 amino acids, the CRISPR-associated protein CasX is a fundamentally distinct RNA-guided nuclease when compared to Cas9 and Cas12a. Although it can induce RNA-guided genome editing in mammalian cells, the activity of CasX is less robust than that of the widely used S. pyogenes Cas9.

Nov 13, 2018 · To establish the immune system, bacteria integrates viral DNA at the proximal end of a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) locus, and the foreign DNAs are expressed and processed into CRISPR RNA (crRNA) in response to subsequent encounters with viral DNAs.

CHyMErA is suitable for the high-throughput deletion of genetic segments including the excision of individual exons. Furthermore, CHyMErA enables the concomitant targeting of two or more genes and can thus be used for the systematic mapping of genetic interactions in …

Apr 10, 2019 · Here we describe CHIME: CHimeric IMmune Editing, a CRISPR-Cas9 bone marrow delivery system to rapidly evaluate gene function in innate and adaptive immune cells in vivo without ex vivo manipulation of these mature lineages.

Aug 20, 2023 · In this review, we will discuss both the current scientific and legal landscape of human–animal chimera (HAC) research.

Oct 17, 2013 · Sequence-specific control of gene expression on a genome-wide scale is an important approach for understanding gene functions and for engineering genetic regulatory systems. We have recently...