JC-1 staining of breast cancer cells after DOX, PD, and DOX + PD treatments. MCF-7 and MDA-MB-231 cells were treated with 5 μM DOX, 10 μM PD, or 5 μM DOX + 10 μM PD. After 4 h of...

May 10, 2018 · We assessed mitochondrial membrane potential through staining with JC1 where the ratio of red (JC1 aggregate): green (JC1 monomer) depicts mitochondrial viability.

May 17, 2014 · JC1 and H2AX immunofluorescence were used to assess mitochondrial and nuclear DNA damage, respectively. This study demonstrated that AG has a dose-dependent …

JC-1 mitochondrial membrane potential assay is a fluorescence-based method to track the behavior of mitochondria. Mitochondrial membrane potential (MMP) is an important parameter …

The polyphenol epigallocatechin-3-gallate (EGCG) in combination with doxorubicin (Dox) exhibits a synergistic activity in blocking the growth and colony-forming ability of human prostate cell …

Mitochondrial membrane potential of the cardiomyocytes was measured by JC-1, an indicator mitochondrial function, in cardiomyocytes treated with Ber (1.0 µM) or/and DOX (1.0 µM) for 1 …

Ratiometric JC-1 imaging reliably unveiled differences in mitochondrial shape, ΔΨ m, and apparently, also the sensitivity to metabolic challenge of individual mitochondria in their cellular …

Feb 25, 2021 · We assessed the potential of DOX–Tf conjugate as a drug delivery system, monitoring its cytotoxicity using the MTT assay and ATP measurements. Moreover, we …

The microscopic images demonstrate the effect of DOX and its complexes on the mitochondrial membrane potential (Δψm), only for the cells treated with DOX-1IOV.

JC-1 has been difficult to dissolve, but this kit solves the problem! HeLa cells treated with depolarizing reagent, carbonylcyanide-p-trifluoromethoxyphenylhydrazone (FCCP) were …

Nov 22, 2012 · JC-1 fluorescence is usually excited by the 488 nm laser wavelength common in flow cytometers. In this study, we show that in practice this approach is not optimal for …

Apr 6, 2017 · After a 5 hour long treatment, cells were loaded with the voltage—sensitive fluorescent mitochondrial dye—JC1 –then red and green fluorescence was determined by …

JC-1, a cationic fluorescent dye when added to living cells, is known to be localized exclusively in mitochondria, particularly in good physiological conditions characterized by sufficient …

Mar 7, 2012 · When K562/Dox cells are transfected with empty vector (K562/Dox‐H1), PgP expression is sustained at levels similar to the parental K562/Dox cells, confirming the …

Dec 1, 2018 · SRB assay unveiled the antiproliferative activity of Quer on breast cancer cell line MCF7 at 72 h. Quer enhanced the antiproliferative effect of Dox in MCF7 cells and as …

Download scientific diagram | Mitochondrial membrane potential (MMP) measurement by JC1 dye on (a) MDA-MB 468 (b) 4T1 cells (c), (d) quantification data of MMP in MDA-MB 468 and 4T1 …

Isogenic disomic clone that spontaneously lost one chromosome 21 after electroporation was isolated and served as a control for expression of the dox control element in the disomic ± dox …

Our results reveal a new role of Fe-MIL-101 as a novel, non-toxic anti-angiogenic agent that restricted ovarian tumour growth. These findings could open a new avenue of using MOFs as …

May 12, 2024 · Our study explores the direct effects of doxorubicin (DOX) exposure on microRNA (miRNA) expression and some putative molecular targets to elucidate the cellular and …