Background Gap junction channels (GJCs) are massive protein channels connecting the cytoplasm of adjacent cells. These channels allow intercellular transfer of molecules up to ~1 kDa,...

Jan 1, 1992 · A three-dimensional model derived from the X-ray structure to simulate electron microscope images has proven useful in interpreting the variations among Fourier averaged connexon images (Sosinsky et al., 1986).

Aug 8, 1977 · X-ray crystallographic methods and electron microscope image analysis have been used to correlate the structure and the chemical composition of gap junction plaques isolated intact from mouse liver.

Fluorescence microscopy allows localization of specific proteins to junctions, but with a resolution on the same scale as junction size, rendering internal protein organization unobtainable.

Jan 17, 2017 · Early electron microscopy images of gap junction channels. a Cellular membrane between two rat liver cells. b Cellular membrane of rat liver cells immediately after isolation exhibiting the characteristic hexagonal pattern.

Gap junctions between identified cells are visualized by transmission electron microscope (TEM) in the RC1 data set and after re-imaging at 0.3 nm resolution. Panels A-G are native Viking...

Oct 31, 2024 · In this Review, we explore new insights from studies using super-resolution and volume electron microscopy into the nanoscale organization of these junctional complexes as well as the roles of the junction-associated cytoskeleton, neighboring …

Gap junctions were originally defined based on structure (appearance in electron micrographs) 4– 6. They also were among the first membrane channels for which low-resolution three-dimensional structural analyses were accomplished.

Jan 11, 2021 · (B–D) Electron micrographs of a gap junction plaque (B), an invaginated gap junction (C) and a connexosome (D). Gap junctions are labeled with an anti-Cx43 antibody and 10 nm-gold-conjugated secondary antibody.

Although described in some detail by light microscopy in the 19th century, it was in 1966 that electron microscopy images showed that the ID represented apposing cell borders, and provided...