Jul 14, 2021 · Here we designed and genetically encoded a new latent bioreactive Uaa, fluorosulfonyloxybenzoyl-l-lysine (FSK), in E. coli and mammalian cells. Due to its long and …

We designed mFSY to complement FSY and FSK and further expand covalent targeting abilities (Fig. 1a). While FSK has a long and flexible side chain to reach target residues at further …

Jun 7, 2023 · Moreover, we established the use of FSK and FSY for genetically encoded chem. crosslinking to capture elusive enzyme-substrate interactions in live cells, allowing us to target …

FSK complements FSY to expand target diversity and versatility. Together, they provide a powerful, genetically encoded, latent bioreactive SuFEx system for creating covalent bonds in …

Lei Wang is the winner of the 2021 Emil Thomas Kaiser Award. To expand protein's covalent bonding ability, latent bioreactive unnatural amino acids have been designed and genetically …

Here we designed and genetically encoded a new latent bioreactive Uaa, fluorosulfonyloxybenzoyl- l -lysine (FSK), in E. coli and mammalian cells.

Sep 18, 2024 · Research laboratory of Lei Wang, PhD Chemical Biology Laboratory We genetically encode unnatural amino acids in live cells and organisms to investigate biological …

fluorosulfonyloxybenzoyl-L-lysine (FSK) in E. coli and mammalian cells. 23 FSK has similar reactive specificity as FSY, but it contains a longer and more flexible side chain

In this review, we briefly summarize residue selective chemical crosslinking, with reactivity activated by light or by proximity. We focus on small molecule crosslinkers and genetically …

W Wang, JK Takimoto, GV Louie, TJ Baiga, JP Noel, KF Lee, ... ‪Professor, UCSF. The Salk Institute for Biological Studies (prior)‬ - ‪‪Cited by 18,136‬‬ - ‪Chemical Biology‬.