May 15, 2023 · H H1299 cells were treated with Etoposide, Etoposide plus NEDD4L (WT) cDNA and Etoposide plus NEDD4L (S448A) cDNA. Cell viability assay showed that NEDD4L cDNA significantly decreased H1299...

Jun 12, 2023 · Cell viability analyses revealed that the half-maximal inhibitory concentration (IC 50) of etoposide for etoposide-resistant (Eto-R) H1299 cells increased by 30-fold (16.31 μM to 0.57 μM) (Fig....

Download scientific diagram | Etoposide cytotoxicity in H1299 cells is antagonized by p53 and p21 waf1 expression. C-30 cells and W-65 cells were seeded at 5610 5 cells/100 mm dish.

In H1299 cells, VP-16-induced cytotoxic effect was demonstrated associated with apoptosis that disappeared when restored with wild-type p53. Cell cycle analysis revealed that, upon VP-16 induction, cell death began with growth arrest by accumulating cells at the G (2)-M phase.

Jan 16, 2025 · We experimentally confirmed the activation of senescence in A549 and H1299 lung cancer cells following treatment with 0.5 or 5 μM etoposide (Fig. 5 F and G and Figs. S6 J and L to N).

Sep 14, 2022 · In our experiments, when H1563 and H1299 cell cultures in serum-free BM or NDM were treated with cisplatin or etoposide, we found no direct linkage between LD formation and cancer cell sensitivity to a specific chemotherapeutic.

Aug 6, 2006 · To determine whether SirT1 regulates E2F1-mediated apoptosis after DNA damage, H1299 SirT1 -knockdown cells were treated with etoposide and analysed for cell death by MTT assay.

Jun 1, 2024 · Enhanced ferroptosis, inhibition of A549 and H1299 cell proliferation, and migration of the NPs. Apoptosis was investigated by biochemical staining and flow cytometry. Etoposide (EPT) offers significant benefits in the tumor therapy domain.

Jan 18, 2015 · Induction of p53 and its target gene p21 was detected by etoposide in A549 cells but not in H1299 cells. However, PPE8 could not stimulate p53 and p21 induction in both A549 and H1299 cells (Figure 6 (a)).

(A) Clones of H1299 cells were pre-treated with 100 μM IPTG to permit expression of the wild type, S15A or S15D p53 proteins. The cells were then treated for up to 6 h with 50 μM etoposide and...