Mar 1, 2023 · To analyse this in more detail, we determined H3K4me3 and RNAPII location using ChIP–seq analysis of DPY30–mAID cells after RA treatment with or without auxin treatment.

Sep 14, 2016 · Here, by using a small-scale chromatin immunoprecipitation followed by sequencing (ChIP–seq) method 3, we map the genome-wide profiles of histone H3 lysine 4 trimethylation (H3K4me3) and...

May 6, 2022 · To accurately compare H3K4me3 ChIP-seq signals at different time points between WT and spp1-W45A, we added Schizosaccharomyces pombe cells into each sample as spike-in.

Sep 2, 2014 · Here we present a new method that implements a microfluidic device to facilitate the ChIP process, providing a technology to obtain the high-quality ChIP-Seq data from merely 1 000 mammalian...

Jul 8, 2021 · A meta-analysis of H3K4me3 ChIP-seq data across > 20 different cell and tissue types in mice and humans, revealed a subset of genes marked by a broad H3K4me3 domain spanning the TSS. In identifying genes with the broad H3K4me3 domain, typically ChIP-seq peaks are called using the broad-peak function in MACS2 [ 9 , 42 , 43 , 44 ].

Histone H3K4me3 ChIP-seq analysis in Mll2 ΔSET ESCs revealed loss of H3K4me3 at sites distinct from those seen in Set1BKO-Set1A ΔSET when compared with WT cells (fig. S2, C and H).

Dec 1, 2014 · Typical ChIP-seq experiments with TF antibodies show a distribution that closely resembles that of random fragments from the genome. Gene proximal areas typically have higher GC-content and this is reflected in the H3K4me3 samples that …

Jul 15, 2021 · In this study, we demonstrated high levels of histone H3 lysine K4 trimethylated (H3K4me3) and dysregulation of DNA transcription in circulating neutrophils of HIV-infected subjects. This dysregulation was accompanied by a deficient response of neutrophils to LPS, impaired cytokine/chemokine/growth factor synthesis, and increased apoptosis.

Feb 10, 2023 · The combined analysis of ChIP-seq and RNA-seq data supports that H3K4me3 coincides with actively transcribed genes and H3K27me3 is associated with repressed genes that show low levels of transcription.

In the current study, ChIP-Seq data corresponding to histone modification H3K4me3 for one normal-like (MCF10A) and four breast cancer cell lines (luminal-A [MCF7, ZR751], TNBC [MB231, MB436]) were chosen to understand role of miRNA-gene …