The Homologous to E6AP C-terminus (HECT) family E3 ubiquitin ligases are a kind of E3 ubiquitin ligases with a C-terminal HECT domain that mediates the binding of ubiquitin to substrate proteins and a variable-length N-terminal extension.

Homologous to E6AP C-terminus (HECT) E3 ubiquitin ligases play a critical role in various cellular pathways, including but not limited to protein trafficking, subcellular localization, innate immune response, viral infections, DNA damage responses and apoptosis.

Jul 30, 2021 · Thanks to their multi-functions, E3 ligases can be a promising target of cancer therapy. A deeper understanding of the regulatory mechanisms of E3 ligases in tumorigenesis will help to find new prognostic markers and accelerate …

Apr 2, 2019 · In this review, we focus on the HECT-containing E3 ligases. Invariably at their C-terminus, all HECT E3s present the catalytic HECT domain, composed of a bulkier N-terminal lobe (N-lobe) that contains the E2 binding domain, and a C-terminal lobe (C-lobe) carrying the catalytic cysteine.

In this review, we focus on the HECT-containing E3 ligases. Invariably at their C-terminus, all HECT E3s present the catalytic HECT domain, composed of a bulkier N-terminal lobe (N-lobe) that contains the E2 binding domain, and a C-terminal lobe …

Feb 1, 2012 · For the E3 ligases of the homologous to the E6AP carboxyl terminus (HECT) domain family (Rotin and Kumar, 2009), this involves an obligate thioester intermediate with the active-site cysteine of the E3.

May 13, 2009 · Ubiquitylation, or tagging proteins with ubiquitin, is a post-translational modification that targets proteins for degradation by the 26S proteasome or to other cellular fates, such as altered...

Jun 30, 2020 · HECT ubiquitin ligase plays an essential role in the development of many human diseases. In this review, we focus on the physiological and pathological processes involved in oxidative stress and the role of E3 ubiquitin ligase of the HECT family.

Apr 3, 2019 · These characteristics made HECT E3s intriguing targets in drug discovery in the context of cancer biology. Keywords: E3 ligase; HECT regulations; cancer; inhibitor; ubiquitin. Ubiquitination plays a pivotal role in several cellular processes and is critical for protein degradation and signaling.

Stringent control of HECT E3 ligase activity and substrate specificity is essential for cellular health, whereas deregulation of HECT E3s plays a prominent role in disease. The cell employs a wide variety of regulatory mechanisms to control HECT E3 activity and substrate specificity.