HK2-NaCl cells showed a perinuclear distribution of VIM compared to a uniform cytosolic organization of the VIM filaments in HK2-STD cells. Figure 3. Differential expression of VIM forms under osmotic stress conditions in HK2renal epithelial cells.

Download scientific diagram | In human HK2 renal tubular cells, CsA induces both TGFb1- independent loss of E-cadherin and ZO-1 and de novo synthesis of vimentin. HK2 cells were treated for...

Aug 10, 2021 · Our results showed that knockdown of HK2 significantly inhibited vimentin and Twist1 expression and promoted TJP1 and E‐cadherin expression in CRC cells. Additionally, transcriptional and enzymatic inhibition of HK2 by 3‐bromopyruvate (3‐bp) impaired oxaliplatin resistance in vitro and in vivo.

Sep 26, 2023 · We investigated gene and protein expression of HK-2 cells cultured in six medium formulations. TGF-β1 increased the expression of ACTA2, TGFB1, COL4A1, EGR2, VIM and CTGF genes while reducing PPARG in all medium formulations.

(A-C)The HK-2 cells under 30 mM glucose (B) or 30 mM glucose+cont siRNA (C) showed a loss of CK and E-cadherin and an increase of a-SMA and vimentin expression compared to that of the cells...

Oct 22, 2019 · Knockdown of ETS2 abrogated TGF-β1-mediated expression of profibrogenic factors vimentin, α-SMA, collagen I, and FN in HK2 cells. Mechanistically, ETS2 promoted JUNB expression in HK2 cells...

Sep 16, 2014 · With regard to colorectal cancer (CRC), we studied two critical gate enzymes, hexokinase 2 (HK2), which is involved in glycolysis, and phosphorylated pyruvate dehydrogenase-E1α (p-PDH), which is involved in oxidative phosphorylation (OxPhos).

Jan 1, 2010 · Overexpression of URG11 in a human proximal tubule cell line (HK2) promoted a mesenchymal phenotype accompanied by reduced expression of the epithelial marker E-cadherin and increased expression of the mesenchymal markers vimentin and α-SMA, while URG11 knockdown by siRNA effectively reversed hypoxia-induced EMT.

Download scientific diagram | Expression levels of ZEB1, ZEB2 and vimentin in HK-2 cells after exposure to extrinsic free miRNAs for 24 hours. from publication: Non-vesicle-bound free microRNAs...

Mar 1, 2016 · PA treatment was associated with increased angiotensin II levels in cultured medium. The angiotensin II type 1 receptor (AT 1 R) blocker valsartan or renin inhibitor aliskiren dramatically suppressed PA-induced upregulation of BiP, CHOP, IRE1α, p …