Jul 10, 2012 · WT and KO fibroblasts were exposed to stressors that induce hsp70 including heat shock, cadmium, and azetidine-2-carboxylic acid (AZE), as well as 2-deoxyglucose (2-DG), a stressor that does not routinely induce hsp70.

Hsp70 (encoded by three very closely related paralogs: HSPA1A, HSPA1B, and HSPA1L) is a stress-induced protein. High levels can be produced by cells in response to hyperthermia, oxidative stress, and changes in pH. Binding immunoglobulin protein (BiP or Grp78) is a protein localized to the endoplasmic reticulum. It is involved in protein ...

We describe the de novo design of Hsp70 binding proteins that either inhibit or stimulate Hsp70 ATPase activity. An ATPase stimulating design promoted the refolding of denatured luciferase in vitro, similar to native JDPs.

Hsp70 proteins are central components of the cellular network of molecular chaperones and folding catalysts. They assist a large variety of protein folding processes in the cell by transient association of their substrate binding domain with short ...

Ability of Hsp70 domains to bind and stabilize endogenous VEGFA mRNA. A, Western blots of Hsp70 in whole cell lysates from HeLa cells transfected with siHsp70 versus a control siRNA (siC) and rescue by cotransfection of pCMV-FLAG-Hsp70sm, encoding an siRNA-resistant FLAG-Hsp70wt mRNA.

Heat shock proteins (HSPs) are induced in response to many injuries including stroke, neurodegenerative disease, epilepsy, and trauma. The overexpression of one HSP in particular, Hsp70, serves a protective role in several different models of ...

Oct 29, 2021 · Hsp70 transport mechanisms were studied by analyzing its intracellular tracks with Rab proteins as well as by using specific inhibitors of membrane domains.

Jun 28, 2019 · This knowledge showcases how the Hsp70 chaperone system controls diverse cellular functions, and offers new opportunities for the development of chemical compounds that modulate disease-related...

Jan 28, 2020 · Hsp70-enriched exosomes derived from heat-treated CT26 mouse colon carcinoma cells significantly enhanced antitumor effect of TDEs via the expression of MHC II in an in vitro antigen presenting cell model.

Mar 19, 2015 · Affinity chromatography provides evidence that Az binds HSP70 but not other types of heat shock proteins including HSP40, HSP60, and HSP90. We also demonstrate that Az induces cancer cell death via caspase-dependent apoptosis by disrupting the interaction of HSP70 with APAF-1.