A recently described automated microparticle enzyme immunoassay (Abbott IMx CA 125) for cancer antigen 125 (CA 125) was recalibrated by the manufacturer to provide better agreement between the results of the IMx assay and other test methods for CA 125.

The IMx assay detected as little as 0.193 kAU/L CA 125 (AU = arbitrary Abbott unit), demonstrated up to 29% overestimation upon serum dilution, low within-assay (2.7-5.6%) and between-assay (4.8-8.2%) CVs, and no high-dose hook effect < or = 46,000 kAU/L nor influence from human anti-mouse antibodies in serum of women injected with OC 125 F(ab')2.

We compared the improved Abbott IMx cancer antigen including most of the clinical studies mentioned above, (CA) 125 assay (cat. no. 7A89) with the Abbott CA 125 have been performed with the first commercially avail-RIA.

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