Track mitochondrial membrane potential with JC-1 dye. Flow Cytometry & Imaging kits. Detect membrane potential in a variety of cell types and in intact tissues.

Nov 22, 2012 · JC-1 fluorescence is usually excited by the 488 nm laser wavelength common in flow cytometers. In this study, we show that in practice this approach is not optimal for monitoring...

The following protocol describes the preparation steps for the use of the JC-1 dye on cultured cells in Suspension followed by analysis of the cells preparation by flow cytometry, fluorescence microscopy or fluorescence plate reader.

Cells labeled with JC-1 can be analyzed by flow cytometry using 488 nm excita-tion and green or orange-red emission, and by fluorescence microscopy using standard filters for Alexa Fluor ® 488 dye and R-phycoerythrin.

The MitoProbe™ JC-1 Assay Kit provides a mitochondrial membrane-potential disrupter, CCCP, in order to produce compensation controls leading to a correctly compensated green-to-red fluorescence ratio.

Feb 12, 2025 · JC-1 dye, a cationic carbocyanine dye, selectively permeates mitochondria, making it invaluable for assessing mitochondrial membrane potential. Its molecular structure, characterized by a delocalized positive charge, allows it to accumulate within the mitochondria in a potential-dependent manner.

JC‐1 Mitochondrial Membrane Potential Assay comprise of lipophilic cationic fluorescent dye; 5,5,6,6’‐tetrachloro‐1,1’,3,3’‐tetraethylbenzimi‐dazoylcarbocyanine iodide (JC‐1). In healthy cells, JC‐1 enters the energized mitochondria and form aggregates which change the fluorescent property of JC‐1 dye.

Distinguish high and low mitochondrial membrane potential in living cells using JC-1 (Iodide) dye. JC-1 offers a fast and convenient staining protocol that is well suited for high-throughput screening and compatible with flow cytometry applications. JC-1 is a lipophilic, cationic carbocyanine dye.

Bivariate JC-1 analysis of mitochondrial membrane potential in HL60 cells by flow cytometry. The sensitivity of this technique is demonstrated by the response to K+/valinomycin-induced depolarization (panel B).

Cayman’s JC-1 Mitochondrial Membrane Potential Flow Cytometry Assay Kit can be used to study mitochondrial behavior in a variety of conditions, including apoptosis. Changes in Δψ M, reflected by aggregation level of JC-1, can be determined as a ratio of red to green mean fluorescence intensities using flow cytometry. Flow cytometry is an ...