Oct 23, 1996 · Cytotoxic lymphocytes utilize granule associated serine proteases (granzymes) and perforin to induce apoptosis. Although the importance of granzyme B has been …

Importantly, Jurkat T cells incubated with granzyme B and a sublytic concentration of perforin undergo apoptosis, which is preceded by the activation of endogenous ICE-LAP3. Thus, we …

As shown in Fig. 8A, when Jurkat T cells were activated with PHA plus PMA, granzyme B transcription was up-regulated. In contrast, exposure to the NF-B inhibitors PDTC and SSZ …

Jurkat cells overexpressing Bcl-2 or transfected with vector backbone DNA only (Jurkat-pgk) were exposed for the times indicated to granzyme B (60 nM) and sublytic PLO. Control cells were …

Feb 1, 2004 · Moreover, apoptosis of Jurkat cells was induced, consistent with the observation that the PMN lysates contain enzymatically active granzyme B. Taken together, our data …

Using a Jurkat cell line as a model, we examined how overexpression of Bcl-2 influenced mitochondrial membrane potential, intracellular caspase activation, and DNA fragmentation …

Jurkat and Jurkat stably expressing Bcl-2 were treated with 1 μg/ml granzyme and adenovirus for 3 h in the absence or presence of 100 μ m zVAD-fmk. Cell lysates were collected, analyzed by …

Sep 15, 2007 · To probe this issue in further detail, we examined intracellular protease activity in Jurkat cell targets overexpressing Bcl-2. The Jurkat Bcl-2 transfectants used have significantly …

Apr 1, 2006 · To address whether NF-κB signaling also plays a regulatory role for granzyme B transcription in activated T cells, Jurkat T cells were stimulated with PHA plus PMA for 2 h in …

Aug 1, 1998 · We also studied the ability of granzyme B alone to trigger apoptosis in target cells. Jurkat, treated with granzyme B, were labeled by the TUNEL technique to assess DNA …