Tyrosine kinase inhibitor (TKI) resistance is a major problem in chronic myeloid leukemia (CML). We generated a TKI-resistant K562 sub-population, K562-IR, under selective imatinib-mesylate...

The leucocyte-specific phosphatase CD45 is present in two main isoforms: the large CD45RA and the short CD45RO. We have recently shown that distinctive expression of these isoforms distinguishes natural killer (NK) populations.

Does anyone have experience with the markers expressed on K562 cells? I have checked DSMZ and when I stain my cells with the markers stated that K562 should be positive for and run the profile...

Feb 27, 2020 · CD45, CD33, CD65, CD146 hematologic marker expressions are reduced in K562-IR cells. CD44 and SSEA-4 have increased expression while no difference was observed in CD34 and CD38. Grey area in the histograms is background signal from isotype control.

Sep 28, 2022 · To explore how these human CD45-NKR molecules impact NK cell activity, we co-cultured human NK cells from human peripheral blood mononuclear cells (PBMCs) with target cells expressing human HLA ligands and found that the CD45–Z270 significantly enhanced the killing of K562-GpHLA-E cells (Figure 7G).

ADOPT can acquire immunofluorescent 3D volumetric data from suspended cells with isotropic resolution. We investigated its performance to quantify the presence of CD45 markers on the surface of K562 cells.

Materials and Methods: Characteristics of six human leukemia cell lines –Kasumi-1, NB-4, MOLM-13, MV-4-11, K562, and Jurkat cells– were investigated using multiple color analysis of surface...

Nov 24, 2015 · In K562 cells, CD45 expression decreased to around 58% of control, indicating that the surface proteins are highly stable and this limits the achievable maximum knockdown (data not shown).

We observed a tenfold increase in the enucleation rate compared with the control, an increase in the production of hemoglobin, and expression of the erythroid marker CD71 coupled with a decrease in the lymphoid marker CD45.

Complete karyotype characterization of the K562 cell line by combined application of G-banding, multiplex-fluorescence in situ hybridization, fluorescence in situ hybridization, and comparative genomic hybridization.

Aug 14, 2023 · Here, we have characterized K562 cells focusing on the karyotype of cells in prolonged culture, regulation of expression of TfR in wildtype (WT) and doxorubicin-resistant cells, and responses to histone deacetylase inhibition (HDACi).

Here, we have characterized K562 cells focusing on the karyotype of cells in prolonged culture, regulation of expression of TfR in wildtype (WT) and doxorubicin-resistant cells, and responses to histone deacetylase inhibition (HDACi).

Apr 21, 2016 · The leucocyte-specific phosphatase CD45 is present in two main isoforms: the large CD45RA and the short CD45RO. We have recently shown that distinctive expression of these isoforms distinguishes natural killer (NK) populations.

Feb 27, 2020 · The expression of CD45 (leukocyte common antigen-LCA, the general marker for hematopoietic cells) is significantly reduced in resistant cells; suggesting that K562-IR cells are gradually drifting away from their hematological origin.

See Pross, et al. for a detailed analysis of the in vitro assay of NK cells including the mathematics of quantitation of NK cell activity. The continuous cell line K-562 was established by Lozzio and Lozzio from the pleural effusion of a 53-year-old female with chronic myelogenous leukemia in terminal blast crises.

Feb 27, 2020 · We generated a TKI-resistant K562 sub-population, K562-IR, under selective imatinib-mesylate pressure. K562-IR cells are CD34-/CD38-, BCR-Abl-independent, proliferate slowly, highly adherent and form intact tumor spheroids. Loss of CD45 and other hematopoietic markers reveal these cells have diverged from their hematopoietic origin.

TNF-α-mediated CD45 and CD30 shedding is shown to be to be time- and dose-dependent and associated with significant increase in LDH release, with maximal effects after 24 h of treatment. The percentage of decrease of all examined cell surface molecules on K-562 cells after TNF-α treatment was not uniform and appeared to depend on the ...

These changes (Table 2) included a loss of granularity of the cells, a progressive loss of CD45 expression (CD45 expression is low in erythroid and platelet precursors and high in cells of the...

We found that K562 expressing CMV-pp65, CD40L and OX40L effectively stimulates CMV-specific responses in vitro by promoting antigen cross-presentation to professional antigen-presenting cells (APCs). Vaccination also enhances antitumor effects of CAR-redirected CMV-CTLs in xenograft tumor models.

May 19, 2002 · In K562 cells, p53-mediated caspase activation per se did not result in an apoptotic response, but appeared to play a role during the differentiation process. Indeed, the expression of CD45 and GlyPhA markers, upregulated by wt-p53, were reduced after treatment with the caspase inhibitor zVAD-fmk.