CellLight Mitochondria-GFP, BacMam 2.0, provides an easy way to label mitochondria with green fluorescent protein (GFP) in live cells. Simply add the reagent to your cells, incubate overnight, and the cells are ready to image in the morning.

In this chapter, we detail the use of advanced confocal microscopy and mitochondrial matrix-targeted photoactivatable green fluorescent protein (mito-PAGFP) for the investigation of mitochondrial dynamics. We focus on direct visualization and quantification of mitochondrial fusion and mitochondrial network complexity in living mammalian cells.

Jan 1, 2001 · This chapter describes green fluorescent protein (GFP) marker proteins that are correctly targeted to different mitochondrial compartments: the matrix, the inner and outer membranes, and mitochondrial DNA (mtDNA) in cells of …

PAGFP can be targeted to different parts of the mitochondrion in order to examine the mixing properties that underlie fusion events between these organelles. Once targeted, spatially precise photoconversion can be used to induce GFP fluorescence.

Sep 9, 2019 · Here we engineered MitoTag mice, which express a Cre recombinase-dependent green fluorescent protein targeted to the outer mitochondrial membrane, and developed an isolation approach to profile...

May 1, 2000 · Three facts are apparent when viewing mitochondria expressing GFP: (i) mitochondrial are not distributed evenly within the cytoplasm; (ii) mitochondria are not all the same size or shape; and (iii) mitochondria can be highly dynamic structures, changing shape and position within a second.

Purpose: We describe a novel strategy for expression of GFP in mammalian mitochondria. Methods: The key components of the strategy were an artificially created mitochondrial genome pmtGFP and a DQAsome transfection system.

Jul 20, 2012 · Mitochondrial fusion plays an essential role in mitochondrial calcium homeostasis, bioenergetics, autophagy and quality control. Fusion is quantified in living cells by photo-conversion of matrix targeted photoactivatable GFP (mtPAGFP) in a subset of mitochondria.

4 days ago · Confocal microscopy revealed that GFP-labeled exogenous mitochondria were detected within PCs in the 1st week post-transplantation and remained intact for up to 4 weeks (Supplementary Fig. 11c).

Jul 26, 2024 · We made use of a recently developed Bi-genomic split-GFP assay (BiG Mito-Split-GFP, hereafter shortened as BiG Mito-Split) where a larger fragment of GFP, GFP 1-10 is encoded in the mitochondrial DNA, and the small fragment GFP 11 is fused with a nuclear-encoded protein (Bader et al, 2020).