Multi-color live-cell STED nanoscopy of mitochondria with a ... - PNAS
Dec 19, 2022 · Using multi-color STED microscopy, we demonstrate that imaging with PKMO can capture interactions of mitochondria with different cellular components such as the …
Multicolour nanoscopy of fixed and living cells with a single STED …
Apr 18, 2017 · To date, up to four markers had been distinguished in STED experiments featuring comparatively elaborate imaging schemes and optical setups, and exploiting various …
Multicolor Stimulated Emission Depletion (STED) Microscopy to …
In this protocol, we describe how to fix, permeabilize, immunostain, and mount RSV-infected A549 cells for STED imaging. We show that STED increases resolution compared to confocal …
Whole-Cell, 3D, and Multicolor STED Imaging with Exchangeable ...
Dec 10, 2018 · We demonstrate that exchangeable labels can achieve high labeling density, circumvent fluorophore bleaching, and allow for whole-cell, multicolor, and live-cell STED …
Multi-color live-cell STED nanoscopy of mitochondria with a
Dec 19, 2022 · Using multi-color STED microscopy, we demonstrate that imaging with PKMO can capture interactions of mitochondria with different cellular components such as the …
Multicolour Multilevel STED nanoscopy of Actin/Spectrin ... - Nature
May 25, 2016 · Superresolution fluorescence microscopy of multiple fluorophores still requires development. Here we present simultaneous three-colour stimulated emission depletion …
STED microscopy - Wikipedia
Multicolor STED was developed in response to a growing problem in using STED to study the dependency between structure and function in proteins. To study this type of complex system, …
Multiplexed stimulated emission depletion nanoscopy (mSTED) for …
Jul 5, 2024 · However, multi-color STED imaging in live-cell remains challenging due to the limited excitation wavelengths and large amount of laser radiation. Here, we develop a multiplexed …
Dynamic Monitoring of Organelle Interactions in Living Cells via …
Jan 7, 2025 · One of the most significant advances in stimulated emission depletion (STED) super-resolution microscopy is its capacity for dynamic super-resolution imaging of living cells, …
Protein‐Specific, Multicolor and 3D STED Imaging in Cells with …
Oct 11, 2019 · Here, we demonstrate confocal and STED microscopy with short, fluorophore-labeled oligonucleotides that transiently bind to complementary oligonucleotides attached to …