Jun 1, 1982 · Chromatography on BND_cellulose can be used for purification and concentration of DNA eluted from agarose gels. Double-stranded linear or circular DNA is eluted from BND-cellulose with 20% ethanol in 1 M NaCl. The recovery depends on the molecular sized of …

Un-nicked circular DNA can be separated from protein, RNA, and other DNA in a simple three-step protocol consisting of exonuclease III digestion, extraction with benzoylated naphthoylated DEAE-cellulose (BND cellulose) in 1 M NaCl, and alcohol precipitation of the remaining supercoiled DNA.

Jul 23, 2005 · BND-Cellulose Absorption Numbers: 5% of total DNA are single stranded. 0.2ml of packed BND-cellulose ( about 0.1g ) absorb 10ug of ssDNA.(use 0.5 to 0.6ml(2ml sln) for 1 to 2x10^8 c.e.) For 50 to 100ug DNA prep., we use 0.5ml of packed BND-cellulose (about 0.25g) Buffers: BND Wash: 5M NaCl, 10mM Tris (pH8.0), 1mM EDTA

Jan 1, 1992 · In this report we deal with the distribution among photosynthetic organisms of both NADP+-dependent G3P DHases, namely phosphorylating (chloroplastic) and non-phos- phorylating (cytosolic), in a number of higher plants and strains representative of the main tax- onomic groups of microalgae.

Naci Bnd is on Facebook. Join Facebook to connect with Naci Bnd and others you may know. Facebook gives people the power to share and makes the world more open and connected.

consisting of exonuclease III digestion, extraction with benzoylated naphthoylated DEAE-cellulose (BND cel-lulose) in 1 M NaCI, and alcohol precipitation of the remaining supercoiled DNA....

May 1, 1985 · Un-nicked circular DNA can be separated from protein, RNA, and other DNA in a simple three-step protocol consisting of exonuclease III digestion, extraction with benzoylated naphthoylated...

Feb 1, 1979 · We show in this report that the DNA-protein complex specifically binds to benzoylated naphthoylated DEAE-cellulose (BND-cel- lulose) because of the protein component, and demonstrate the presence of a protein on the ends of replicating adenovirus DNA.

Jan 1, 2009 · Here, we present a detailed step-by-step protocol for RIP mapping of replication origins in budding yeast. Origins of DNA replication are the chromosomal regions from which bidirectional duplication of the genome initiates.

Jan 1, 2009 · Owing to the high magnification that can be achieved, transmission electron microscopy (EM) has helped uncovering the fine architecture of DNA replication forks and improved our understanding of the physiological process of genomic or episomal DNA duplication, in a number of different systems ranging from bacteriophages to mammalian cells (1 – 3).