The OGDH E1-TPP mechanism involves the formation of a stabilized carbanion intermediate. Three classes of these multienzyme complexes have been characterized: one specific for pyruvate, a second specific for 2-oxoglutarate, and a third specific for branched-chain α …

Apr 30, 2022 · Here, we report the 2.92 Å resolution cryo-electron microscopy (EM) structure of its E1 component 2-oxoglutarate dehydrogenase (OGDH). The density map comprised residues 129–1,023, which is nearly the full length of OGDH. The structure clearly shows the active site and Ca2+ binding site of OGDH.

Alpha-ketoglutarate dehydrogenase also known as 2-oxoglutarate dehydrogenase E1 component, mitochondrial is an enzyme that in humans is encoded by the OGDH gene. [5] [6] [7]

Sep 27, 2024 · Moreover, we analyzed and validated the interactions between each subunit, and determined the flexible binding mode for E1o, E2o and E3, resulting in a proposed model of Sus scrofa OGDHc....

Here, we show that loss of DHTKD1 in glutaryl-CoA dehydrogenase-deficient HEK-293 cells leads to a 2-fold decrease in the established GA1 clinical biomarker glutarylcarnitine and demonstrate that oxoglutarate dehydrogenase (OGDH) is responsible …

2-oxoglutarate dehydrogenase (OGDH) is the first rate-limiting E1 subunit of OGDH complex (OGDHC), which plays as a regulatory point in the cross-road of TCA cycle and glutamine metabolism. Until now, the role of OGDH in carcinogenesis has been unclear.

Jul 7, 2023 · In the second decarboxylation step, the OGDH complex, which is composed of three subunits (OGDH/E1, DLST/E2 and DLD/E3), converts oxoglutarate into succinyl-CoA, which is converted to succinate by succinyl-CoA synthetase.

Pyruvate dehydrogenase (PDH), branched-chain 2-oxo acid dehydrogenase (BCDH) and 2-oxoglutarate dehydrogenase (OGDH) are multienzyme complexes that play crucial roles in several common metabolic pathways.

OGDH complex comprises three components, 2-oxoglutarate dehydro- genase (E1 OGDH), dihydrolipoamide succinyltransferase (E2 DLST), and dihydrolipoamide dehydrogenase (E3 DLDH), carrying out the coupled reaction to decarboxylate α-ketoglutarate to succinyl-CoA.

The three component enzymes of the OGDH assembly function in series: E1o is a ThDP-dependent 2-oxoglutarate decarboxylase, E2o a lipoylated succinyltransferase and E3 is a dihydrolipoyl dehydrogenase.