In this chapter, we present applications of FTIR for the structural analysis of RNA, including the analysis of helical parameters and noncanonical base pairing, often found in RNA. The effect of temperature pretreatment, which has a great impact on RNA folding, will also be discussed.

Complementing the cellular results with the FTIR analysis of extracted RNA, ds-DNA, ss-cDNA and isolated nuclei, we verified that the spectral component centered at ∼1220 cm –1 is a good qualitative and semiquantitative marker of cellular DNA, since it …

The database contains an extensive collection of Fourier Transform Infrared (FTIR) spectra for a wide range of nucleic acid molecules, including DNA, RNA, and their derivatives.

FT-IR spectra of uridine in CsI matrix recorded (from top to bottom) at +150 °C, +50 °C and −180 °C, respectively. The spectra of the RNA nucleosides are presented with wavelength (μm) on the x-axis, aligning with the format preferred by astrophysicists and consistent with …

Apr 29, 2021 · Extending this principle, non-coding RNAs can potentially serve as therapeutic targets, and new classes of RNA-binding antiviral and anti-cancer drugs can be developed, with FTIR serving as a powerful analytical tool.

Aug 26, 2005 · We introduce time-resolved infrared spectroscopy as a powerful method to study the kinetics of RNA folding and unfolding transitions. A laser-induced temperature jump is used to initiate a perturbation in the RNA structure.

In this chapter, we present applications of FTIR for the structural analysis of RNA, including the analysis of helical parameters and noncanonical base pairing, often found in RNA. The effect of temperature pretreatment, which has a great impact on RNA folding, will also be discussed.

Feb 1, 2020 · In this chapter, we present applications of FTIR for the structural analysis of RNA, including the analysis of helical parameters and noncanonical base pairing, often found in RNA.

This volume looks at the different spectroscopic and biophysical methods used by researchers to study the structure and folding of RNA, and interactions. Chapters cover topics such as single-molecule spectroscopy of multiple RNA species; surface plasmon resonance; FTIR, SAXS and SANS; and CryoEM.

Feb 1, 2020 · In this chapter, we present applications of FTIR for the structural analysis of RNA, including the analysis of helical parameters and noncanonical base pairing, often found in RNA. The effect of temperature pretreatment, which has a …