Jan 20, 2024 · Studies have shown that the SIRT1-BMAL1 pathway can regulate oxidative stress. To investigate its correlation with oxidative stress, we examined the expression levels and influencing factors of...

6 days ago · BMAL1, as part of the BMAL1/CLOCK complex, binds to the E-box elements in the SIRT1 promoter, and BMAL1/CLOCK may directly regulate SIRT1 expression by binding to E-boxes in the SIRT1 promoter 45.

Moreover, CLOCK and BMAL1 regulate the expression of sirtuin 1 (SIRT1), an important regulator of insulin sensitivity, in C2C12 myotubes and mouse skeletal muscle, and two E-box elements in Sirt1 promoter are responsible for its CLOCK- and BMAL1-dependent transcription in …

Our results demonstrate the effects of BMAL1 on estrogen synthesis in hGCs and suggest a BMAL1-SIRT1-JNK positive feedback cycle in this process, which points out an important role of BMAL1 in the development of PCOS.

Jul 25, 2008 · Here, we show that SIRT1, an NAD(+)-dependent protein deacetylase, is required for high-magnitude circadian transcription of several core clock genes, including Bmal1, Rorgamma, Per2, and Cry1. SIRT1 binds CLOCK-BMAL1 in a circadian manner and promotes the deacetylation and degradation of PER2.

Jun 15, 2015 · Exposure to low-dose palmitate suppresses the circadian oscillations of clock genes. Palmitate treatment causes destabilization of BMAL1-CLOCK interaction. SIRT1 activator restores BMAL1-CLOCK interaction and clock gene expression in palmitate-treated hepatocytes.

Increased NAMPT-dependent NAD + production enhances SIRT1 deacetylase activity, which acts to inhibit the transcription of NAMPT through interactions with the CLOCK:BMAL1 complex at the NAMPT promoter, thus completing a feedback loop involving NAMPT/NAD + /SIRT1 and CLOCK:BMAL1.

This study focused on the expression of SIRT1-BMAL1 and risk factors during different onset times of ischemic stroke and revealed that SIRT1-BMAL1 may contribute to the early onset of ischemic stroke by regulating oxidative stress.

Sep 25, 2014 · In the present study, we explored the regulatory effects of SIRTUIN1 (SIRT1), an NAD + -dependent deacetylase, on CLOCK/BMAL1-mediated clock gene expression. To investigate the interaction between SIRT1 and CLOCK/BMAL1, we conducted bimolecular fluorescence complementation (BiFC) analyses supplemented with immunocytochemistry assays.

Jul 7, 2008 · We show that the HDAC activity of the NAD + -dependent SIRT1 enzyme is regulated in a circadian manner, correlating with rhythmic acetylation of BMAL1 and H3 Lys9/Lys14 at circadian promoters. SIRT1 associates with CLOCK and is recruited to the CLOCK:BMAL1 chromatin complex at circadian promoters.