Thiolases, also known as acetyl-coenzyme A acetyltransferases (ACAT), are enzymes which convert two units of acetyl-CoA to acetoacetyl CoA in the mevalonate pathway.

Thiolases are CoA-dependent enzymes that catalyze the thiolytic cleavage of 3-ketoacyl-CoA, as well as its reverse reaction, which is the thioester-dependent Claisen condensation reaction. Thiolases are dimers or tetramers (dimers of dimers).

Thiolase is a conserved enzyme present in the three domains of life (Bacteria, Archaea and Eukarya). This ubiquitous enzyme catalyzes the reversible thiolytic cleavage of 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA, a two-step reaction involving a covalent intermediate formed with a catalytic cysteine.

Thiolase enzymes are essential in cellular metabolism, particularly in the breakdown and synthesis of fatty acids. They catalyze the cleavage and formation of carbon-carbon bonds, vital for energy production and storage.

Sep 22, 2015 · Here the authors uncover a redox-switch within Clostridium acetobutylicum’s thiolase—a key enzyme involved in n-butanol biosynthesis—that controls the rate of fermentative butanol production.

Thiolases are a well characterized family of enzymes with two distinct categories: degradative, β-ketoadipyl-CoA thiolases and biosynthetic, acetoacetyl-CoA thiolases. Both classes share an identical catalytic triad but catalyze reactions in opposite directions.

Dec 30, 2024 · Thiolase is essential in the first step of synthesizing many value-added biochemicals. However, only a few feasible THLs have been reported, and a limited understanding of these enzymes has restricted their broader …

Mar 22, 2022 · Thiolase plays important roles in lipid metabolism. It can be divided into degradative thiolases (thioase I) and biosynthetic thiolases (thiolases II), which are involved in fatty acid β-oxidation and acetoacetyl-CoA biosynthesis, respectively.

Oct 2, 2020 · Thiolase are ubiquitous enzymes that catalyze the reversible thiolytic cleavage of 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA, a 2-step reaction involving a covalent intermediate formed with a catalytic cysteine. They are found in prokaryotes and eukaryotes (cytosol, microbodies and mitochondria).

Thiolase superfamily is an important class of enzymes that belong to CoA dependent enzyme group. These enzymes are involved in fatty acid biosynthesis by carbon-carbon bond formation via a thioester-dependent Claisen-condensation reaction.