Jan 22, 2025 · In this study, pathway reconstruction and integration site optimization of Saccharomyces cerevisiae enabled the production of 363.0 mg/L of vanillin in just four steps, …

Jan 1, 2024 · Vanillin biosynthesis is necessary to meet the growing demand for natural vanillin. Biotransformation pathways of microbes are inadequate for high vanillin synthesis. …

Jan 5, 2015 · The natural vanillin biosynthesis pathway in the vanilla orchid, V. planifolia, has an elegant solution to cope with the toxicity issue by glucosylation of vanillin to vanillin-β-d …

Jun 19, 2014 · Here we show that a single hydratase/lyase type enzyme designated vanillin synthase (Vp VAN) catalyses direct conversion of ferulic acid and its glucoside into vanillin and …

Mar 29, 2017 · The present review compiles the up-to-date knowledge on vanillin biosynthesis in plant systems to focus principally on the enzymatic reactions of in planta vanillin biosynthetic …

The present review compiles the up-to-date knowledge on vanillin biosynthesis in plant systems to focus principally on the enzymatic reactions of in planta vanillin biosynthetic pathway and to …

Dec 14, 2022 · Through integrating sam8, CYP199A2, comt, fcs and ech into the genome of S. cerevisiae, vanillin was successfully biosynthesized with a titer of 26.3 μg/L. Preferred sam5 …

Vanillin, a common flavoring agent, is produced from ground black vanilla bean pods. Vanillin can also be produced from the biodegradation of lignin, a paper manufacturing byproduct and also …

pathway was constructed in Escherichia coli cells, where cheap and accessible carbon sources (e.g., glucose, glyc-erol, and xylose) were used as primary precursors for vanillin production. …

Mar 18, 2025 · The vanillin biosynthesis pathway has not been thoroughly deciphered and multiple hypotheses are considered. A comparative transcriptomic approach between two …