More importantly, a pivotal protein, SIRT1, was involved in this process by specifically up-regulating ADAM10 and down-regulating BACE1, which is dependent on the cAMP/PKA pathway in APP/PS1 primary neurons and SH-SY5Y cells …

Dec 1, 2021 · Here, we provide a mechanistic connection between PKA signaling and SIRT1 that directly affects nutrient selection/utilization and total energy expenditure under both pharmacological adrenergic activation and the well-established, physiological adaptive thermogenic response.

Oct 30, 2019 · We report, for the first time, that PBMT activates SIRT1 via the cAMP/PKA pathway. Importantly, the current study found that the mitochondrial photoacceptor CcO is responsible for PBMT‐induced activation of PKA and SIRT1.

Dec 23, 2011 · The NAD(+)-dependent deacetylase SIRT1 is an evolutionarily conserved metabolic sensor of the Sirtuin family that mediates homeostatic responses to certain physiological stresses such as nutrient restriction.

In response to β-adrenergic signaling, the cAMP-dependent protein kinase (PKA) pathway activates SIRT1 to promote downstream oxidative metabolism . SIRT1 is also highly regulated by the concentration of its substrate NAD + , thereby coupling energy status to SIRT1 activity.

SIRT1 S434 in in vitro kinase assays (Figure S3B), suggesting that other kinases, downstream of PKA, might be responsible for the direct phosphorylation of SIRT1 in vivo.

However, work in HepG2 cells suggested that PKA, not SIRT1, was key to hepatic gluconeogenesis . In the present study, we dissected potential cellular pathways by which PKA reduces cytoplasmic HMGB1. We used REC grown in normal (5mM) and high glucose (25mM) with a PKA agonist, SIRT1 antagonist, and IGFBP-3 and Epac1 siRNA. We hypothesized that ...

However, here we have identified a cAMP-induced phosphorylation of a highly conserved serine (S434) located in the SIRT1 catalytic domain that rapidly enhanced intrinsic deacetylase activity independently of changes in NAD+ levels.

Photoactivation of mitochondrial photoacceptor cytochrome c oxidase (CcO) is responsible for the activation of PKA/SIRT1 signaling pathway.

Dec 23, 2011 · Here, we provide a mechanistic connection between PKA signaling and SIRT1 that directly affects nutrient selection/utilization and total energy expenditure under both pharmacological adrenergic activation and the well-established, physiological adaptive thermogenic response.